Graphene multi-transistor array chips as molecular biosensors

by Dr Pedro Alpuim (International Iberian Nanotechnology Laboratory, Centro de Física das Universidades do Minho e do Porto)

Wednesday 10 May 2023, 14:00 → 15:00 Portugal

Sala de Conferências (Departamento de Física FCTUC)

As real-life applications loom, graphene and graphene-based materials are approaching the industrial production stage [1]. Upscaled graphene and 2D materials growth processes can be bottom-up and top-down, such as chemical vapor deposition (CVD) and liquid phase exfoliation (LFE). While CVD is particularly suitable for high-mobility single-layer graphene for micro and nanoelectronic devices, LFE methods allow for directly depositing multi-layered films at low cost over large-area substrates, like those used in macroelectronics [2]. In this presentation, we report biosensing chips based on CVD graphene field-effect transistor (GFET) microelectronic arrays capable of detecting dopamine in attomolar concentrations in mice brain samples [3] or discriminating between different Port wine grapevine varieties based on the number of SNPs that are present in the studied portion of their genome, again with an attomolar limit of detection [4].

In a different approach, graphene is used as a sensing platform for super-resolution microscopy and bio-sensing by resonance energy transfer between DNA origami labeled with a fluorophore, immobilized on the graphene surface. A Fluorescence Imaging (FLIM) optical microscopy setup is used for imaging [5].

References

[1] W. Kong, H. Kum, H., S. Bae, et al., Nat. Nanotechnol. 14 927 (2019).

[2] S. Tkachev, M. Monteiro, et al., Adv. Funct. Mater..2103287 (2021).

[3] M. Abrantes, et al., J. of Nanobiotechnology 20 (1) 495 (2022).

[4] A. Purwidiantri et al., ACS Sensors 8 (2) 640-654 (2023).

[5] R.M.R. Adão et al., 2D Materials 6 (4), art. no. 045056 (2019).